High Voltage Study #2

This High Voltage study was with NON-CONTACT corona discharge, which means a high voltage of at least 100,000-200,000 volts. What this in vitro study found was that high voltage corona discharge, like seen in the BELS multiwave oscillators, significantly improved electroporation in the cells. Electroporation, as the name signifies, uses electric fields to induce pores or permeability in the cells. This allows for greater delivery of nutrients and better removal of wastes.

The study (which you can download below), specifically looked at the effects of non-contact corona discharge and the improved delivery of a chemotherapy drug in one trial and plasmid DNA in another. The conclusion was a statistically significant reduction in tumor size on the one hand, and an increased gene expression on the other.

Only one machine (the BELS Biocharger) on our reviews will allow the user to adjust the output voltage from 100,000 volts to 750,000 volt and create a significant corona for effective electroporation. Other units produce no corona discharge which means low voltage, around 100,000 volts and very little (if any electroporation).

The TRUTH is higher voltage is more HEALING.

Download the study below or check out the Abstract (below the download).

highvoltagestudy2.pdf
Download File

Corona Ion Deposition: A Novel Non-Contact Method for Drug and Gene Delivery to Living Systems

Niraj Ramachandran ABSTRACT

Application of corona ions produced in air to B16F10 murine melanoma cells in vitro and in animal models resulted in the transport of molecular therapeutics across the cell membrane. This work presents the development of new methods for drug and gene delivery based upon similar principles as the traditional electrode driven membrane destabilization processes known as electroporation. This was achieved with non-contact corona ion deposition that temporarily increased the permeability of cell membranes.

Interaction of corona charge with biological cells was studied and their potential for molecular delivery was established. Molecular delivery was first demonstrated in vitro using tracer molecules followed by in vitro delivery of the cytotoxic drug bleomycin. Building upon these results, the delivery of bleomycin coincident with ion deposition was shown to significantly slow the growth of very aggressive solid tumors in animal models, compared to drug alone or no treatment. Delivery of plasmid DNA to cells in the skin of animal models indicated that application of corona ions (both positive and negative) to live tissue pro- duced a four to six fold increase in gene expression. As this is the first significant study of the interaction and impact of corona ions on the delivery of drug and plasmid DNA to biological cells, numerous fundamental investigations were performed and discussed. A charge dose dependence was observed and physical mechanistic models were proposed. A model of cell resealing time constant following corona ion exposure was developed and demonstrated a reasonable prediction of experimental findings. The foundation laid by this work may enable continued exploration and use of corona ion deposition in the future as a new and promising physical method for drug and gene delivery.